anti-cd137 (17b5  (Thermo Fisher)

Journal: International Journal of Biological Sciences

Article Title: CD137L-macrophage induce lymphatic endothelial cells autophagy to promote lymphangiogenesis in renal fibrosis

doi: 10.7150/ijbs.66781

Figure Lengend Snippet: CD137 expression correlated with intrarenal lymphangiogenesis and fibrosis in IgAN. (A) IHC analysis of CD137 expression (40×); Masson's trichome staining showing collagen deposition(40×); (B) Representative images and double-positive analyses for immunofluorescence-labeled D2-40 (green) and CD137 (red) in IgA patients (n=85) and controls (n=5). Scale bar, 20µm. (C) Relationships between CD137 + lymphatic vessels in the interstitium and Serum urea nitrogen, Serum Creatinine, Serum uric acid, proteinuria, Plasma albumin, HB and eGFR. HB, hemoglobin; eGFR, estimated glomerular filtration rate; (D) The difference of density of lymphatic vessels and CD137 + lymphatic vessels between patients with different T and S grades. T, tubular atrophy/interstitial fibrosis; S, segmental glomerulosclerosis.

Article Snippet: The renal fibrosis model was induced by unilateral ureteral obstruction (UUO) as previously reported. anti-CD137 blocking antibody (4-1BB, Clone:17B5, Bio X Cell, Lebanon NH) were injected (i.p.) at 10 mg/kg/dose twice weekly.

Techniques: Expressing, Staining, Immunofluorescence, Labeling, Clinical Proteomics, Filtration

Journal: International Journal of Biological Sciences

Article Title: CD137L-macrophage induce lymphatic endothelial cells autophagy to promote lymphangiogenesis in renal fibrosis

doi: 10.7150/ijbs.66781

Figure Lengend Snippet: Demographic and clinical parameters between patients with D2-40 and CD137 + D2-40 expression.

Article Snippet: The renal fibrosis model was induced by unilateral ureteral obstruction (UUO) as previously reported. anti-CD137 blocking antibody (4-1BB, Clone:17B5, Bio X Cell, Lebanon NH) were injected (i.p.) at 10 mg/kg/dose twice weekly.

Techniques: Expressing, Clinical Proteomics

Journal: International Journal of Biological Sciences

Article Title: CD137L-macrophage induce lymphatic endothelial cells autophagy to promote lymphangiogenesis in renal fibrosis

doi: 10.7150/ijbs.66781

Figure Lengend Snippet: Histopathologic features from renal biopsies between patients with low or high levels of CD137 + D2-40 expression.

Article Snippet: The renal fibrosis model was induced by unilateral ureteral obstruction (UUO) as previously reported. anti-CD137 blocking antibody (4-1BB, Clone:17B5, Bio X Cell, Lebanon NH) were injected (i.p.) at 10 mg/kg/dose twice weekly.

Techniques: Expressing

Journal: International Journal of Biological Sciences

Article Title: CD137L-macrophage induce lymphatic endothelial cells autophagy to promote lymphangiogenesis in renal fibrosis

doi: 10.7150/ijbs.66781

Figure Lengend Snippet: CD137 is increased in the obstructed kidney. (A) IHC staining showing LYVE-1 and CD137 expression in the sham control and UUO mice (400×). The expression levels of Prox-1 and LYVE-1 were detected by Western blot (B), and the expression levels of CD137 were detected by real-time PCR (C). (D) Immunofluorescence staining showing CD137 (red) expression in UUO kidneys in the interstitium on days 7 and 14 and colocalization with LYVE-1 (green) original magnification, ×400. n = 6 per group. The error bars represent the SEM. ***P < 0.001.

Article Snippet: The renal fibrosis model was induced by unilateral ureteral obstruction (UUO) as previously reported. anti-CD137 blocking antibody (4-1BB, Clone:17B5, Bio X Cell, Lebanon NH) were injected (i.p.) at 10 mg/kg/dose twice weekly.

Techniques: Immunohistochemistry, Expressing, Control, Western Blot, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining

Journal: International Journal of Biological Sciences

Article Title: CD137L-macrophage induce lymphatic endothelial cells autophagy to promote lymphangiogenesis in renal fibrosis

doi: 10.7150/ijbs.66781

Figure Lengend Snippet: Demographic and clinical parameters between patients with low or high levels of CD137 + D2-40 expression.

Article Snippet: The renal fibrosis model was induced by unilateral ureteral obstruction (UUO) as previously reported. anti-CD137 blocking antibody (4-1BB, Clone:17B5, Bio X Cell, Lebanon NH) were injected (i.p.) at 10 mg/kg/dose twice weekly.

Techniques: Expressing, Clinical Proteomics

Journal: iScience

Article Title: Induction of Foxp3 and activation of Tregs by HSP gp96 for treatment of autoimmune diseases

doi: 10.1016/j.isci.2021.103445

Figure Lengend Snippet:

Article Snippet: CD137-PE/Cy7 (17B5) , eBioscience , Cat#25-1371-80;RRID: AB_2573397.

Techniques: Recombinant, SYBR Green Assay, Cell Isolation, Staining, Plasmid Preparation, Software